Protein Chaperones and Protein Translocases

            My lab has an opening for recent PhD graduates or fellows with less than 2 years PDF experience for postdoctoral positions starting early 2010. The research focuses on the system specific chaperones in bacteria that are responsible for the maturation of various membrane associated redox enzymes that utilize the Twin arginine translocation (Tat) system.   This system moves folded proteins across the cell membrane. 

            Projects centre on DmsD, the system specific chaperone for DMSO reductase, as well as related chaperones. The substrate DmsABC goes through a complicated pathway to reach maturation and cofactor insertion. The chaperone is thought to escort the enzyme substrate through the steps of maturation.  During this pathway the complex interacts with other chaperones and cofactor biosynthetic pathways as well as the Tat translocase itself.  We are interested in the complete interactome of this chaperone and its substrates protein complex DmsAB.   We utilize biophysical approaches (Fluorescence, calorimetry, BiaCore, etc) with molecular microbiology methods (two hybrid approaches, mutagenesis) with protein chemistry (Protein purification, cross-linking, molecular probes) to study the molecular mechanism of interaction between the chaperones and their substrates and accessory protein complexes.  Many specific projects exist within this context.

            Candidates should have a strong CV with evidence of research and writing ability of publications in internationally recognized quality journals.  Suitable candidates will be sent further information on specific projects.  It would be wise, however, for candidates to explore the labs interest and progress in the field (http://people.ucalgary.ca/~turnerr) to describe how their background or interests are key to the project. Preferences will be given to candidates with experience in the field, or experience in techniques advantageous to the project such as in vitro transcription – translation, protein cross-linking, Co-IP, and other protein-protein interaction methods. A strong background in protein chemistry or protein physical chemistry is desirable. However, a molecular microbiologist with knowledge of proteins could carry some projects out well. Fellows in my group are expected to contribute to the training and supervision of junior researchers (undergraduates and junior graduate students).  Opportunities for contributing to undergraduate course lectures will be made available if interested to facilitate experience for career.  Salaries will be based on experience, granting council (CIHR) and university guidelines. Information on University of Calgary and helpful resources to PDF’s at the university can be found at www.ucalgary.ca/postdoc. 

            The Turner group is a protein biochemistry and microbial physiology lab with interests in bacterial systems.  The group is well established with 1-3 PDF’s, 2-5 graduate students, and 2-3 undergrads at any given time.   To express your interest in this position send CV and cover letter of project interest to:

Professor Raymond J. Turner, PhD

Dept. of Biological Sciences,

156 Biological Sciences Building,

University of Calgary, 2500 University Dr. N.W.

Calgary, Alberta, Canada, T2N 1N4

Tel:403-220-4308            Fax:403-289-9311

E-mail:turnerr@ucalgary.ca

http://homepages.ucalgary.ca/~turnerr

 

 

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Protein Chaperones and Protein Translocases

     My lab has openings for graduate student positions starting early 2010.  The research focuses on the system specific chaperones in bacteria that are responsible for the maturation of various membrane associated redox enzymes that utilize the Twin arginine translocation (Tat) system.   This system moves folded proteins across the cell membrane. 

     Projects centre on DmsD, the system specific chaperone for DMSO reductase, as well as related chaperones. The substrate DmsABC goes through a complicated pathway to reach maturation and cofactor insertion. The chaperone is thought to escort the enzyme substrate through the steps of maturation.  During this pathway the complex interacts with other chaperones and cofactor biosynthetic pathways as well as the Tat translocase itself.  We are interested in the complete interactome of this chaperone and its substrates protein complex DmsAB.   We utilize biophysical approaches (Fluorescence, calorimetry, BiaCore, etc) with molecular microbiology methods (two hybrid approaches, mutagenesis) with protein chemistry (Protein purification, cross-linking, molecular probes) to study the molecular mechanism of interaction between the chaperones and their substrates and accessory protein complexes.  Many specific projects exist within this context.

     Positions are available to start January, May or Sept 2010.  The Department of Biological Sciences, faculty of Science requires a minimum of 3.2/4.0 gpa for consideration for graduate program.  Successful candidates should have a M.Sc. degree in a relevant area or a gpa of >3.6/4.0.  Students will participate as Teaching assistants and/or compete for scholarship support to supplement their stipend. Students are expected to participate in training and working with others by supervising junior students.  For further information on the department’s graduate student policies see www.bio.ucalgary.ca/graduate/index.html.

     The Turner group is a protein biochemistry and microbial physiology lab with interests only in bacterial systems.  The group is well established with 1-3 PDF’s, 2-5 graduate students, and 2-3 undergrads at any given time.   To express your interest in this position contact:

Professor Raymond J. Turner, PhD

Dept. of Biological Sciences,

156 Biological Sciences Building,

University of Calgary, 2500 University Dr. N.W.

Calgary, Alberta, Canada, T2N 1N4

Tel:403-220-4308   Fax:403-289-9311

E-mail:turnerr@ucalgary.ca

http://homepages.ucalgary.ca/~turnerr

  

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Technician Position Available

Ottawa Institute of Systems Biology, Baetz Lab

 

We are looking for a dedicated and conscientious Technician to oversee the daily running of the Baetz Lab and to perform basic yeast manipulations/experiments on a variety of functional genomic yeast projects. The ideal candidate would have extensive laboratory experience in:

            -     Media Making

-       Yeast genetics and basic yeast manipulation.

-       Molecular genetics and plasmid construction, including PCR skills, point mutagenesis,

-       Protein purification

-       Performing co-immunoprecipitations

-       Performing enzymatic assays, (eg kinase or KAT assay)

-       Fluorescent Microscopic experience

-       Purchasing in an academic setting

 

Importantly we are looking for a highly organized and motivated individual who deeply cares about the success of their own research and those around them.  A “team player” is a must in our collaborative environment. 

 

Compensation will be competitive.

 

If you think this is the position for you, please send your cover letter and CV, along with contact information for 3 references to Kristin Baetz, kbaetz@uottawa.ca