Canadian Biomolecular Science Information’s goal is to give access to good quality methods of characterization of biological molecules catering to universities and industries. We offer protein characterization, Circular Dichroism, and Analytical Ultracentrifugation–with corresponding service fees.
- This involves expression and purification requiring recombinant proteins. We offer services that reproduce an extrachromosomal DNA for E. coli bacteria that use up to 4 strains of expression with BL21(DE3). This adds to the cell’s toxicity, resulting in a multiplication of protein soluble expressions.
- Plasmid production: cell supply or plasmid supply
- Small range solubility viewing device
- Our instrument has an absorption and wavelength spectropolarimeter that measures a polarized light’s properties. It can be used to assess and monitor the stability of proteins thermally and chemically.
This determines the following:
- Substances’ optical pureness
- Secondary and tertiary build of nucleic acid and protein
- Figure stability at different temperature, pH, and unnatural concentrations
- Figurative changes due to molecular mutualism
- Figuration of proteins in varying systems
- Stable thermodynamics constants
- Folding and unfolding movements of large molecules
- Avoid buffers in the spectral zone.
- The general requirement of sodium and potassium buffer should be 10mM. The pH of sulfuric and phosphoric acid is checked when using Tris of TEA.
- MOPS, DTT, chloride, imidazole and citrate should be avoided. Most buffers target shorter wavelengths where the important structural information is found.
- Test a scale of buffer before starting to avoid buffer problems.
- The sample should be free from impure substances that will contribute to the signaling of the CD machine. It is highly recommended for the buffer to undergo dialysis.
- The concentration of the sample is known by the cuvette’s path length. We have a variety of choices
- The sample should not go beyond an absorption rate of 0.9 OD exceeding the wavelengths you want to see. Always measure the absorption rate of your samples in all the wavelengths you want to use to make sure it does not go over the requirement.
- A good estimation of the 190-230nm scale is 0.2mg/ml when you are using a 1mm cuvette.
- Having a smaller path length cuvette will lower down the absorption of the solvents to give way to scanning wavelengths that are low, though it will need more of a concentrated sample.
- From dialysis, bring a minimum of 500uL sample and a minimum of 10mL of a similar buffer.
Analytical Ultracentrifugation (AUC)
- This is a strong method of characterizing solutions of large molecules for analysis: quantifying interactions of macromolecules. Velocity and Equilibrium of sedimentation are the two types of experiments carried out.
AUC applications determine the following:
- The sample’s purity
- Molecular mass
- Oligomer development
- Changes in configuration
Complex atomic and molecular bonding/Ligand bonding